Journal: Molecules (Basel, Switzerland)

Article Title: Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains of Aspergillus flavus .

doi: 10.3390/molecules27227861

Figure Lengend Snippet: Figure 1. MALDI-TOF mass spectra of AFB1 presented as tandem peaks with mass spectrum of 313.150 m/z [M + H]+ and 335.004/335.003 m/z [M + Na]+. Peaks are indicated with arrows.

Article Snippet: After the initial A. flavus (producers and non-producers) mass spectra analysis and selection, they were transferred to the MALDI Biotyper OC software (Bruker Daltonics, Bremen, Germany) and used its i . I- ctr of FB1 isolated directly from wheat and barley grains infected by producing strain (335.219 m/z) and non-producing strain of A. flavus (AFB1 peak absent).

Techniques:

Journal: Molecules (Basel, Switzerland)

Article Title: Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains of Aspergillus flavus .

doi: 10.3390/molecules27227861

Figure Lengend Snippet: Figure 2. MALDI-TOF mass spectra of AFB1 obtained by scraping from TLC plate. Standard AFB1 (335.237 m/z) and producing strain of A. flavus (335.223 m/z).

Article Snippet: After the initial A. flavus (producers and non-producers) mass spectra analysis and selection, they were transferred to the MALDI Biotyper OC software (Bruker Daltonics, Bremen, Germany) and used its i . I- ctr of FB1 isolated directly from wheat and barley grains infected by producing strain (335.219 m/z) and non-producing strain of A. flavus (AFB1 peak absent).

Techniques:

Journal: Molecules (Basel, Switzerland)

Article Title: Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains of Aspergillus flavus .

doi: 10.3390/molecules27227861

Figure Lengend Snippet: Figure 3. MALDI-TOF mass spectra of AFB1 obtained by isolation from Aspergillus flavus mycelia. Standard AFB1 (334.957 m/z), non-producing strain (peak absent) and producing strain with the peak (335.231 m/z).

Article Snippet: After the initial A. flavus (producers and non-producers) mass spectra analysis and selection, they were transferred to the MALDI Biotyper OC software (Bruker Daltonics, Bremen, Germany) and used its i . I- ctr of FB1 isolated directly from wheat and barley grains infected by producing strain (335.219 m/z) and non-producing strain of A. flavus (AFB1 peak absent).

Techniques: Isolation

Journal: Molecules (Basel, Switzerland)

Article Title: Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains of Aspergillus flavus .

doi: 10.3390/molecules27227861

Figure Lengend Snippet: Figure 4. MALDI-TOF mass spectra of AFB1 obtained by isolation from medium around the As- pergillus flavus mycelia. Producing strain (335.231 m/z) and non-producing strain (without peak of alfatoxin B1).

Article Snippet: After the initial A. flavus (producers and non-producers) mass spectra analysis and selection, they were transferred to the MALDI Biotyper OC software (Bruker Daltonics, Bremen, Germany) and used its i . I- ctr of FB1 isolated directly from wheat and barley grains infected by producing strain (335.219 m/z) and non-producing strain of A. flavus (AFB1 peak absent).

Techniques: Isolation

Journal: Molecules (Basel, Switzerland)

Article Title: Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains of Aspergillus flavus .

doi: 10.3390/molecules27227861

Figure Lengend Snippet: Figure 5. MALDI-TOF mass spectra of AFB1 isolated directly from wheat and barley grains infected by producing strain (335.219 m/z) and non-producing strain of A. flavus (AFB1 peak absent).

Article Snippet: After the initial A. flavus (producers and non-producers) mass spectra analysis and selection, they were transferred to the MALDI Biotyper OC software (Bruker Daltonics, Bremen, Germany) and used its i . I- ctr of FB1 isolated directly from wheat and barley grains infected by producing strain (335.219 m/z) and non-producing strain of A. flavus (AFB1 peak absent).

Techniques: Isolation, Infection

Journal: Nature protocols

Article Title: High-throughput matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry-based deubiquitylating enzyme assay for drug discovery.

doi: 10.1038/s41596-020-00405-0

Figure Lengend Snippet: Fig. 3 | HTS MALDI-TOF DUB assay workflow. Incubation time and workflow possible pause points are indicated as clock and hourglass symbols, respectively. The compound library is delivered by using an acoustic liquid-handling system (Stage 1) and pre-incubated with the enzyme of interest for 5–30 min at room temperature (Stage 2). The substrate is added to initiate the reaction (Stage 3), and the reaction is incubated for 30–60 min. The reaction is stopped by adding trifluoroacetic acid (TFA) (Stage 4). The procedure can be paused here for ≤48 h. Assay plates are spotted onto the 1536 AnchorChip MALDI target (Stage 5) and analyzed by MALDI-TOF MS (Stage 6). Data are exported and analyzed for positive hit identification (Stage 7). Intens., intensity.

Article Snippet: ● Rapiflex MALDI-TOF mass spectrometer (Bruker; equipped with Compass software for FlexSeries 2.0) ● FlexControl software (Bruker) ● FlexAnalysis software (Bruker) ● Perkin Elmer Envision 2104 microplate reader ● UW Ultrawave QS18 ultrasonic cleaning bath ● 20 cm × 13 cm × 4 cm stainless steel box (for sonicating the MALDI target) ● FluidX, XRD-384 reagent dispenser ● TTP Labtech Mosquito HTS equipped with five-position plate deck ● 16-channel electronic VIAFLO pipette, 2- to 50-μl volume range and appropriate tips PROTOCOL NATURE PROTOCOLS 10 NATURE PROTOCOLS |www.nature.com/nprot

Techniques: Incubation, Drug discovery